Convenient Nucleic Acid Detection for Tomato spotted wilt virus: Virion Captured/RT-PCR (VC/RT-PCR)

Tomato spotted wilt virus

Tomato spotted wilt virus.

Growers in Georgia first noted problems in their peppers and onions during the last week of March 1989. Thrips populations combined with further field symptoms and the verification of the disease triggered a massive insecticide spray program in Georgia. Monitor was first used in early May followed by combinations of Orthene, Cygon and Thiodan. Most fields had less than 1% infection prompting a ...

Comparison of ELISA and RT-PCR assays for the detection of Tomato spotted wilt virus in peanut

Diagnosis of Tomato spotted wilt virus (TSWV) in peanut can be accomplished by enzyme-linked immunosorbent assay (ELISA) or reverse transcription polymerase chain reaction (RT-PCR) but there has been no report of a direct comparison of the success of the two assays in evaluating infection rates of field-grown peanut. We collected peanut root samples from field-grown plants, 76 in 2006 and 48 in...

Tomato Spotted Wilt Virus on Peanuts

I the last decade, tomato spotted wilt virus (TSWV) has become widely distributed across Alabama on many field, vegetable, and floral crops. About 300 plant species, mainly broadleafs, are hosts of TSWV. In addition to peanut, other important crops grown in the Southeast that are susceptible to this virus include tomato, pepper, potato, cucurbits, lettuce, eggplant, and tobacco. Chrysanthemum, ...

A System to Explore Tomato Spotted Wilt Virus Gene Function

Tomato spotted wilt virus (TSWV) has one negative sense (L) and two ambisense (M and S) RNAs. The lack of a tractable TSWV reverse genetics system has impeded direct demonstration of gene function and prevented the usual types of pathogen characterization. The TSWV NSm (non-structural) protein has been presumed to be the viral movement protein (MP) based on a few lines of indirect evidence. We ...